Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢链霉发酵液与溶酶均成功提取到。

NPC cell line (CNE1) as target,Electroporation was used to transfect EBV-LMP gene and the vector into CNE1 cells.

人高分化鼻咽癌株(CNE1)为对象，采用电穿孔基因转染技术，将重组EBV-LMP表达转染CNE1。

Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

方法利用球孢链霉发酵产生的变溶素来消化双歧杆壁，提取，并与商业化溶酶法比较。